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Title 

Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

Authors 

Min-hee HongJungoh AhnS YooJ HongEun Gyo LeeI YoonJoon Ki JungHong-Weon Lee

Publisher 

Elsevier

Issue Date 

2011

Citation 

Veterinary Microbiology, vol. 148, no. 1, pp. 89-92

Keywords 

Antigenic proteinHaemophilus parasuisReverse vaccinology

Abstract 

Haemophilus parasuis causes contagious porcine Gl?sser's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H parasuis.

ISSN 

0378-1135

Link 

http://dx.doi.org/10.1016/j.vetmic.2010.07.023

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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