상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

A novel bifunctional endo-/exo-type cellulase from an anaerobic ruminal bacterium

Authors 

Kyong Cheol KoY HanJong Hyun ChoiG J KimSeung Goo LeeJae Jun Song

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Applied Microbiology and Biotechnology, vol. 89, no. 5, pp. 1453-1462

Keywords 

Bifunctional endo-/exo-type cellulaseCloningExpressionKorean bovine ruminal bacteriumScreening

Abstract 

An anaerobic microorganism termed AN-C16-KBRB was isolated from the bovine rumen and demonstrated cellulolytic activity on a NB agar plate containing azo-carboxymethyl cellulose. The 16S rRNA gene of the strain was 98% similar to that of Clostridiaceae bacterium SK082 (AB298754) as the highest homology. A novel celEdx16 gene encoding a bifunctional endo-/exocellulase (CelEdx16) was cloned by the shotgun method from AN-C16-KBRB, and the enzyme was characterized. The celEdx16 gene had an open reading frame of 1,104-base pairs, which encoded 367 amino acids to yield a protein of molecular mass 40.4 kDa. The amino acid sequence was 53% identical to that of an endoglucanase from Clostridium thermocellum. CelEdx16 was overexpressed in Escherichia coli and purified using Ni-NTA affinity chromatography. The specific endocellulase and exocellulase activities of CelEdx16 were 15.9 and 3.6 × 10-2 U mg -1, respectively. The Michaelis-Menten constant (K m values) and the maximal reaction velocities (V max values) of CelEdx16 were 47.1 μM and 9.6 × 10-3 μmole min -1 when endocellulase activity was measured and 106.3 μM and 2.1 × 10-5 μmole min-1 when exocellulase activity was assessed. CelEdx16 was optimally active at pH 5.0 and 40°C.

ISSN 

0175-7598

Link 

http://dx.doi.org/1007/s00253-010-2949-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)