상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Detection of low-abundant novel transcripts in mouse hematopoietic stem cells

Authors 

H S KimJ HwangY H KimS KimJ W LeeH S KangK S KimJ H HaJ W ChungKyu Tae ChangZ Y RyooS Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2009

Citation 

Molecular Genetics and Genomics, vol. 282, no. 4, pp. 363-370

Keywords 

Gene expressionGr-1 myeloid progenitor cellHematopoietic stem cellSAGETranscriptome

Abstract 

Gene expression profiles of hematopoietic stem cells (HSCs) provide clues for understanding molecular mechanisms of HSC behavior, including self-renewal and differentiation. We took advantage of serial analysis of gene expression (SAGE) to identify medium- and low-abundant transcripts expressed in HSCs/hematopoietic progenitor cells (HPCs). Among a total of 31,380 unique transcripts, 17,326 (55%) correspond to known genes and, 14,054 (45%) are low-copy transcripts that have no matches to currently known genes. Among the former class, 3,899 (23%) were alternatively spliced transcripts and 3,754 (22%) represent anti-sense transcripts from known genes. Mapping of the SAGE tags to the mouse genome showed that differences in gene expression exist among chromosomes. In addition, comparison of the HSCs/HPCs SAGE data to that of myeloid progenitor cells revealed that massive genetic reprogramming occurs in hematopoietic cell differentiation. Our results demonstrate a previously unrecognized complexity of gene expression in HSCs/HPCs, and indicate the need for further efforts to fully identify and characterize the transcripts expressed in this cell type.

ISSN 

0026-8925

Link 

http://dx.doi.org/10.1007/s00438-009-0469-z

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)