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Title 

Sequential conjugation of 6-aminohexanoic acids and L-arginines to poly(amidoamine) dendrimer to modify hydrophobicity and flexibility of the polymeric gene carrier

Authors 

G S YuH N YuY H ChoeS J SonTai-Hwan HaJ S Choi

Publisher 

The Korean Chemical Society

Issue Date 

2011

Citation 

Bulletin of the Korean Chemical Society, vol. 32, no. 2, pp. 651-655

Keywords 

DendrimerGene deliveryPolyplexTransfection

Abstract 

We synthesized a novel cationic dendrimer consisting of a poly(amidoamine) dendrimer (PAMAM, generation 4) backbone with both L-arginine (Arg) at the termini and 6-aminohexanoic acid (Ahx) between the original core polymer and the peripheral Arg units. The sequential chemical modification of PAMAM G4 with Ahx and Arg resulted in higher transfection efficiency with much less cytotoxicity. PAMAM G4-Ahx-Arg formed stable polyplexes at weight ratios of 8:1 or higher (polymer: plasmid DNA), and the mean polyplex diameter was 180 ± 20 nm. PAMAM G4-Ahx-Arg showed much higher transfection ability than PAMAM G4 or PAMAM G4-Ahx. Furthermore, PAMAM G4-Ahx-Arg was much less cytotoxic than PEI25KD and PAMAM G4-Arg. In addition to Arg grafting of the PAMAM dendrimer, which endows a higher transfection capability, the addition of Ahx spacer increased dendrimer hydrophobicity, introduced flexibility into the conjugated amino acids, and reduced cytotoxicity. Overall, it appears that the concomitant modification of PAMAM with Ahx and Arg could lead to new PAMAM conjugates with better performances.

ISSN 

0253-2964

Link 

http://dx.doi.org/10.5012/bkcs.2011.32.2.651

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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