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Title 

Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea

Authors 

M S U BhuiyanSung Ran MinWon Joong JeongS SultanaK S ChoiW Y SongY LeeY P LimJang Ryol Liu

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Plant Cell Tissue and Organ Culture, vol. 105, no. 1, pp. 85-91

Keywords 

ABC transporterBrassica junceaHeavy metalPhytoremediationYeast cadmium factor 1

Abstract 

A yeast cadmium factor 1 (YCF1) is a member of the ATP-binding cassette (ABC) transporter family associated with multi-drug resistance, and it is localized at the vacuolar membrane in Saccharomyces cerevisiae. To determine ability to increase heavy metal tolerance and accumulation, YCF1 was introduced into Brassica juncea plants by Agrobacterium-mediated genetic transformation. YCF1 gene presence in transgenic plants was demonstrated by polymerase chain reaction (PCR). Reverse transcriptase-PCR analysis confirmed YCF1 gene expression in the transgenic plants, but the degree of YCF1 expression varied among the lines. YCF1 overexpression in B. juncea conferred enhanced tolerance to cadmium (Cd[II]) and lead (Pb[II]) stress. Transgenic B. juncea seedlings showed 1. 3- to 1. 6-fold tolerance to Cd stress and 1. 2- to 1. 4-fold tolerance to Pb stress compared to wild type (WT) plants (per gram fresh weight). Most importantly, the shoot tissues of transgenic seedlings contained about 1. 5- to 2-fold higher Cd(II) and Pb(II) levels than those of WT, demonstrating significantly increased accumulation of both Cd(II) and Pb(II) in transgenic plants.

ISSN 

0167-6857

Link 

http://dx.doi.org/10.1007/s11240-010-9845-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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