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Title 

A novel light-dependent selection marker system in plants

Authors 

Serry KohH KimJ KimE GooY J KimO ChoiN S JwaJ MaT NagamatsuJae Sun MoonI Hwang

Publisher 

Wiley-Blackwell

Issue Date 

2011

Citation 

Plant Biotechnology Journal, vol. 9, no. 3, pp. 348-358

Keywords 

ArabidopsisPhotosensitizerRiceSelection marker systemtflAToxoflavin

Abstract 

Photosensitizers are common in nature and play diverse roles as defense compounds and pathogenicity determinants and as important molecules in many biological processes. Toxoflavin, a photosensitizer produced by Burkholderia glumae, has been implicated as an essential virulence factor causing bacterial rice grain rot. Toxoflavin produces superoxide and H2O2 during redox cycles under oxygen and light, and these reactive oxygen species cause phytotoxic effects. To utilize toxoflavin as a selection agent in plant transformation, we identified a gene, tflA, which encodes a toxoflavin-degrading enzyme in the Paenibacillus polymyxa JH2 strain. TflA was estimated as 24.56kDa in size based on the amino acid sequence and is similar to a ring-cleavage extradiol dioxygenase in the Exiguobacterium sp. 255-15; however, unlike other extradiol dioxygenases, Mn2+and dithiothreitol were required for toxoflavin degradation by TflA. Here, our results suggested toxoflavin is a photosensitizer and its degradation by TflA serves as a light-dependent selection marker system in diverse plant species. We examined the efficiencies of two different plant selection systems, toxoflavin/tflA and hygromycin/hygromycin phosphotransferase (hpt) in both rice and Arabidopsis. The toxoflavin/tflA selection was more remarkable than hygromycin/hpt selection in the high-density screening of transgenic Arabidopsis seeds. Based on these results, we propose the toxoflavin/tflA selection system, which is based on the degradation of the photosensitizer, provides a new robust nonantibiotic selection marker system for diverse plants.

ISSN 

1467-7644

Link 

http://dx.doi.org/10.1111/j.1467-7652.2010.00557.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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