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Title 

Aberrant up-regulation of LAMB3 and LAMC2 by promoter demethylation in gastric cancer

Authors 

Oh Hyung KwonJong Lyul ParkMi-Rang KimJeong Hwan KimH C LeeHee-Jin KimS M NohK S SongHyang Sook YooS G PaikSeon-Young KimYong Sung Kim

Publisher 

Elsevier

Issue Date 

2011

Citation 

Biochemical and Biophysical Research Communications, vol. 406, no. 4, pp. 539-545

Keywords 

DemethylationGastric carcinogenesisMalignant phenotype

Abstract 

The LAMB3 and LAMC2 genes encode the laminin-5 β3 and γ2 chains, respectively, which are parts of laminin-5, one of the major components of the basement membrane zone. Here, we report the frequent up-regulation of LAMB3 and LAMC2 by promoter demethylation in gastric cancer. Gene expression data analysis showed that LAMB3 and LAMC2 were up-regulated in various tumor tissues. Combined analyses of DNA methylation and gene expression of both genes in gastric cancer cell lines and tissues showed that DNA hypomethylation was associated with the up-regulation of both genes. Treatment with a methylation inhibitor induced LAMB3 and LAMC2 expression in gastric cancer cell lines in which both genes were silenced. By chromatin immunoprecipitation assay, we showed the activation histone mark H3K4me3 was associated with the expression of both genes. The expression level of LAMB3 affected multiple malignant phenotypes in gastric cancer cell lines. These results suggest that epigenetic activation of LAMB3 and LAMC2 may play an important role in gastric carcinogenesis.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2011.02.082

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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