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Title 

Redox regulation of the tumor suppressor PTEN by glutaredoxin 5 and Ycp4

Authors 

Y KimK O ChayI KimY B SongT Y KimS J HanY AhnS H ChoKwang Lae HoeB W AhnW K HuhS R Lee

Publisher 

Elsevier

Issue Date 

2011

Citation 

Biochemical and Biophysical Research Communications, vol. 407, no. 1, pp. 175-180

Keywords 

Glutaredoxin 5GlutathioneHydrogen peroxidePTENYcp4

Abstract 

Human PTEN (phosphatase and tensin homolog deleted on chromosome 10; a phosphatidylinositol 3-phosphatase) expressed in Saccharomyces cerevisiae was oxidized in a time- and H2O2-concentration-dependent manner. Oxidized hPTEN was reduced by cellular reductants as in human cells. The reduction rate of oxidized hPTEN was monitored in S. cerevisiae mutants in which the genes involved in redox homeostasis had been disrupted. Reduction of hPTEN was delayed in each of S. cerevisiae grx5Δ and ycp4Δ mutants. Expression of Grx5 and Ycp4 in each of the mutants rescued the reduction rate of oxidized hPTEN. Furthermore, an in vitro assay revealed that the human Grx5/GSH system efficiently catalyzed the reduction of oxidized hPTEN. These results suggest that the reduction of oxidized hPTEN is regulated by Grx5 and Ycp4

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2011.02.133

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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