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Title 

Novel quantitative method for the degree of branching in dextran

Authors 

Young Min KimA KimuraD Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Food Science and Biotechnology, vol. 20, no. 2, pp. 537-541

Keywords 

Degree of branchingDextranEndo-dextranaseExo-dextranase

Abstract 

A novel quantitative method for the determination of degree of branching in Leuconostoc mesenteroides B- 512F dextran was developed by using the combination of 3 dextran-degrading enzymes. First, Paenibacillus sp. endo-dextranase was randomly degraded B-512F dextran into linear or branched isomalto-oligosaccharides with various degree of polymerization (2-8). Second, Streptococcus mutans dextran glucosidase hydrolyzed linear or branched isomalto-oligosaccharides into glucose and branched isomalto-penta-saccharides. Third, the branched isomaltopenta- saccharide was degraded into glucose by using Bacteroides thetaimicron α-glucosidase. The number of branching points in B-512F dextran (5.42%) was determined by the difference in the amount of glucose in the reaction digest between BTGase-PDex and DGase-PDex treatments.

ISSN 

1226-7708

Link 

http://dx.doi.org/10.1007/s10068-011-0075-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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