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Title 

Effect of constitutively active ras overexpression on cell growth in recombinant chinese hamster ovary cells

Authors 

Yeon Gu KimY K HanJ Y KimEun Gyo LeeHong-Weon LeeG M Lee

Publisher 

American Chemical Society

Issue Date 

2011

Citation 

Biotechnology Progress, vol. 27, no. 2, pp. 577-580

Keywords 

Cell growthErythropoietinInducible expressionRasRCHO cells

Abstract 

Constitutively active Ras (CA-Ras) is known to enhance cell growth through the induction of various signaling cascades including the phosphoinositide 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK)/ERK signaling pathways, although the cellular response is highly dependent on the cell type. To evaluate the effect of CA-Ras overexpression on cell growth in recombinant Chinese hamster ovary (rCHO) cells, an erythropoietin (EPO)-producing rCHO cell line with regulated CA-Ras overexpression (EPO-off-CA-Ras) was established using the Tet-off system. The CA-Ras expression level in EPO-off-CA-Ras cells was tightly regulated by doxycycline addition. Although CA-Ras overexpression slightly increased the viable cell concentration during the late exponential phase, it did not increase the maximum viable cell concentration or specific growth rate to a significant degree. Unexpectedly, CA-Ras overexpression in rCHO cells led only to the enhancement in the activation of the MAPK/ERK signaling pathway and not the PI3K/Akt signaling pathway. Taken together, CA-Ras overexpression in rCHO cells did not significantly affect cell growth; it also had no critical impact on viable cell concentration or EPO production, possibly due to a failure to activate the PI3K/Akt signaling pathway.

ISSN 

8756-7938

Link 

http://dx.doi.org/10.1002/btpr.567

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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