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Title 

Genome-wide response of Deinococcus radiodurans on cadmium toxicity

Authors 

M H JoeS W JungS H ImS Y LimH P SongOh Suk KwonD H Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2011

Citation 

Journal of Microbiology and Biotechnology, vol. 21, no. 4, pp. 438-447

Keywords 

CadmiumDeinococcus radioduransIronStress responseTranscriptome

Abstract 

Deinococcus radiodurans is extremely resistant to various genotoxic conditions and chemicals. In this study, we characterized the effect of a sublethal concentration (100 μM) of cadmium (Cd) on D. radiodurans using a whole-genome DNA microarray. Time-course global gene expression profiling showed that 1,505 genes out of 3,116 total ORFs were differentially expressed more than 2-fold in response to Cd treatment for at least one timepoint. The majority of the upregulated genes are related to iron uptake, cysteine biosynthesis, protein disulfide stress, and various types of DNA repair systems. The enhanced upregulation of genes involved in cysteine biosynthesis and disulfide stress indicate that Cd has a high affinity for sulfur compounds. Provocation of iron deficiency and growth resumption of Cd-treated cells by iron supplementation also indicates that CdS forms in iron-sulfur-containing proteins such as the [Fe-S] cluster. Induction of base excision, mismatch, and recombinational repair systems indicates that various types of DNA damage, especially base excision, were enhanced by Cd. Exposure to sublethal Cd stress reduces the growth rate, and many of the downregulated genes are related to cell growth, including biosynthesis of cell membrane, translation, and transcription. The differential expression of 52 regulatory genes suggests a dynamic operation of complex regulatory networks by Cd-induced stress. These results demonstrate the effect of Cd exposure on D. radiodurans and how the related genes are expressed by this stress.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1012.12021

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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