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Title 

Up-regulation and clinical significance of serine protease kallikrein 6 in colon cancer

Authors 

Jong-Tae KimEun Young SongKyung Sook ChungMin A KangJae Wha KimSang Jick KimYoung Il YeomJ H KimK H KimHee Gu Lee

Publisher 

Wiley-Blackwell

Issue Date 

2011

Citation 

Cancer, vol. 117, no. 12, pp. 2608-2619

Keywords 

colon cancerimmunohistochemistryinvasivenesskallikreinkallikrein-related peptidase 6

Abstract 

BACKGROUND: Kallikrein-related peptidase 6 (KLK6) encodes a trypsin-like serine protease that is up-regulated in several cancers, although the putative functions of KLK6 in cancer have not been elucidated. In the current study, overexpression of KLK6 was identified in colon cancer, and the possibility that KLK6 may be a suitable candidate as a tumor marker was examined. METHODS: Messenger RNA (mRNA) transcript levels and protein up-regulation of KLK6 in colon cancer tissues was examined using reverse transcriptase-polymerase chain reaction, immunohistochemistry, and clinicopathologic analyses. Cell proliferation, invasiveness, and antiapoptotic activity were determined in colon cancer cells that were transfected with small-interfering RNA (siRNA) of KLK6. RESULTS: KLK6 mRNA was up-regulated significantly in tumor tissues compared with nontumor regions. KLK6 protein was strongly expressed in adenocarcinomas but was not expressed in normal mucosa or in premalignant dysplastic lesions. Sera from patients with colon cancer revealed an increase in KLK6 secretion (0.25 μg/mL; P =.031) compared with noncancer cells (0.19 μg/mL). Clinicopathologic and immunohistochemical studies of 143 patients with colon cancer revealed a significant correlation between KLK6 expression and Dukes disease stage (P =.005). High KLK6 expression was associated significantly with shorter overall (P =.001) and recurrence-free survival (P =.001). The rates of proliferation and invasiveness were decreased by 50% in cells that were transfected with KLK6 siRNA. The overexpression of KLK6 led to decreased activity of the E-cadherin promoter. CONCLUSIONS: KLK6 was up-regulated significantly in tissues and sera from patients with colon cancer and was associated closely with a poor prognosis, suggesting that KLK6 may be used as a potential biomarker and a therapeutic target for colon cancer.

ISSN 

0008-543X

Link 

http://dx.doi.org/10.1002/cncr.25841

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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