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Title 

VaSpoU1 (SpoU gene) may be involved in organelle rRNA/tRNA modification in Viscum album

Authors 

Joon Woo AhnSuk Weon KimJang Ryol LiuWon Joong Jeong

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Plant Biotechnology Reports, vol. 5, no. 3, pp. 289-295

Keywords 

AdoMet (S-adenosyl-L methionine)MethyltransferaseSpoU familySpoU methylase domainSubcellular localization

Abstract 

The SpoU family of proteins catalyzes the methylation of transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs). We characterized a putative tRNA/rRNA methyltransferase, VaSpoU1 of the SpoU family, from Viscum album (mistletoe). VaSpoU1 and other plant SpoU1s exhibit motifs of the SpoU methylase domain that are conserved with bacterial and yeast SpoU methyltransferases. VaSpoU1 transcripts were detected in the leaves and stems of V. album. VaSpoU1-GFP fusion proteins localized to both chloroplasts and mitochondria in Arabidopsis protoplasts. Sequence analysis similarly predicted that the plant SpoU1 proteins would localize to chloroplasts and mitochondria. Interestingly, mitochondrial localization of VaSpoU1 was inhibited by the deletion of a putative N-terminal presequence in Arabidopsis protoplasts. Therefore, VaSpoU1 may be involved in tRNA and/or rRNA methylation in both chloroplasts and mitochondria.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-011-0178-z

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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