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Title 

Inactivated sendai-virus-mediated fusion improves early development of cloned bovine embryos by avoiding endoplasmic-reticulum-stress-associated apoptosis

 

초기 복제수정란 생산효율을 증가시키는 비활성화된 바이러스 외막 사용에 의한 체세포융합 방법

Authors 

Bong Seok SongJi Su KimSeung Bin YoonKyu-Sun LeeD B KooD S LeeY K ChooJae Won HuhSang Rae LeeSun-Uk KimSang-Hyun KimH M KimKyu Tae Chang

Publisher 

CSIRO Publishing

Issue Date 

2011

Citation 

Reproduction Fertility and Development, vol. 23, no. 6, pp. 826-836

Keywords 

electrofusionsomatic cell nuclear transfer

Abstract 

Somatic cell nuclear transfer (SCNT) is a powerful tool, not only for producing cloned animals, but also in revealing various early developmental events. However, relatively little is known regarding the biological events and underlying mechanism(s) directly associated with early development of SCNT embryos. Here, we show that production of high-quality bovine SCNT blastocysts is dependent on the method used for fusion and the associated reduction in endoplasmic reticulum (ER) stress. During fusion between the donor cell and the enucleated oocyte, electrofusion triggers spontaneous oocyte activation, accompanied by an increase in intracellular Ca2+ and improper nuclear remodelling. These events can be greatly reduced by the use of Sendai virus (SV)-mediated fusion. Moreover, SV-SCNT improves the blastulation rate and blastocyst quality, defined by the number and ratio of inner cell mass and trophectoderm cells in each blastocyst, in comparison with electrofusion- mediated SCNT (E-SCNT). Interestingly, expression of ER-stress-associated genes and blastomere apoptosis were significantly increased in E-SCNT embryos. These increases could be reversed by inhibition of ER stress or by using the SV-mediated fusion method. Collectively, these results indicate that SV-mediated fusion improves the developmental competence and quality of SCNT blastocysts, by reducing ER-stress-associated apoptosis.

ISSN 

1031-3613

Link 

http://dx.doi.org/10.1071/RD10194

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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