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Title 

Stimulation of reductive glycerol metabolism by overexpression of an aldehyde dehydrogenase in a recombinant Klebsiella pneumoniae strain defective in the oxidative pathway

 

글리세롤로부터 13PD, 3-HP의 생산 촉진을 위한 Klebsiella pneumoniae 균주 개량

Authors 

L H LuoJeong-Woo SeoBaek Rock OhP S SeoSun-Yeon HeoWon Kyung HongD H KimChul Ho Kim

Publisher 

Springer

Issue Date 

2011

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 38, no. 8, pp. 991-999

Keywords 

1,3-Propanediol3-Hydroxypropionic acidAldehyde dehydrogenaseGlycerolKlebsiella pneumoniae

Abstract 

Previously, we constructed a glycerol oxidative pathway-deficient mutant strain of Klebsiella pneumoniae by inactivation of glycerol dehydrogenase (dhaD) to eliminate by-product synthesis during production of 1,3-propanediol (1,3-PD) from glycerol. Although by-product formation was successfully blocked in the resultant strain, the yield of 1,3-PD was not enhanced, probably because dhaD disruption resulted in insufficient regeneration of the cofactor NADH essential for the activity of 1,3-PD oxidoreductase (DhaT). To improve cofactor regeneration, in the present study we overexpressed an NAD +-dependent aldehyde dehydrogenase in the recombinant strain. To this end, an aldehyde dehydrogenase AldHk homologous to E. coli AldH but with NAD +- dependent propionaldehyde dehydrogenase activity was identified in K. pneumoniae. Functional analysis revealed that the substrate specificity of AldHk embraced various aldehydes including propionaldehyde, and that NAD + was preferred over NADP + as a cofactor. Overexpression of AldHk in the glycerol oxidative pathway-deficient mutant AK/pVOTHk resulted in a 3.6-fold increase (0.57 g l -1 to 2.07 g l -1) in the production of 3-hydroxypropionic acid (3-HP), and a 1.1-fold enhancement (8.43 g l -1 to 9.65 g l -1) of 1,3-PD synthesis, when glycerol was provided as the carbon source, compared to the levels synthesized by the control strain (AK/pVOT). Batch fermentation using AK/pVOTHk showed a significant increase (to 70%, w/w) in conversion of glycerol to the reductive metabolites, 1,3-PD and 3-HP, with no production of by-products except acetate.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1007/s10295-010-0872-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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