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Title 

Label-free electrochemical detection of human alpha-thrombin in blood serum using ferrocene-coated gold nanoparticles

Authors 

Dohyoung KwonHyun Kyung ChungBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2011

Citation 

Biosensors & Bioelectronics, vol. 28, no. 1, pp. 454-458

Keywords 

AptamerAptasensorElectrochemistryGold nanoparticlesLabel-freeThrombin AptamerThrombin

Abstract 

This paper describes a novel approach to label-free electrochemical detection of human α-thrombin in human blood serum that utilizes ferrocene-coated gold nanoparticles (Fc-AuNPs). Human α-thrombin was specifically bound by the thiolated aptamers immobilized on the electrode. Positively charged Fc-AuNPs were electrostatically bound to the negatively charged aptamers. In principle, a high current peak should be observed in the absence of interactions between the aptamers and the human α-thrombin. This behavior indicates maximum adsorption of Fc-AuNPs by the negatively charged aptamers on the electrode surface. In contrast, when the thrombin-aptamer complex is formed, a low signal is expected because of the blocking capacities of the protein, which hinders the electrostatic binding of the Fc-AuNPs. The electrochemical signal, recorded by cyclic voltammetry and differential pulse voltammetry, indicates whether interactions between aptamers and proteins have occurred. There is a good correlation between the ferrocene oxidation peak intensity readings from our thrombin sensing system and the thrombin concentration, within the range of 1.2 μM-12 pM.

ISSN 

0956-5663

Link 

http://dx.doi.org/10.1016/j.bios.2011.06.040

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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