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Title 

Silver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel

 

Silver-stained fibrin zymography : 단일기질 함유 겔을 이용한 프로테아제의 활성 검증 및 분리

Authors 

Dong Min ChungK E KimKeug Hyun AhnChan Sun ParkD H KimH B KohHyo Kon ChunByung Dae YoonH J KimMin-Soo KimN S Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Biotechnology Letters, vol. 33, no. 8, pp. 1663-1666

Keywords 

ProteaseSilver stainingZymography

Abstract 

A new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed. The method takes advantage of the nanoscale sensitivity of both zymography and silver staining. After SDS-PAGE in a gel containing fibrin, the gel was incubated in enzyme reaction buffer and the zymogram was silver-stained. Bands with protease activity were stained with silver in clear areas where the protein substrate had been degraded. The molecular sizes of proteases were accurately determined. Furthermore, proteases of high molecular weight were clearly and sharply resolved.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1007/s10529-011-0619-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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