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Title 

DNA capturing machinery through spore-displayed proteins

Authors 

T J ParkS J LeeJae Gu PanHeung Chae JungJ Y ParkJ P ParkS Y Lee

Publisher 

Wiley-Blackwell

Issue Date 

2011

Citation 

Letters in Applied Microbiology, vol. 53, no. 4, pp. 445-451

Keywords 

Pathogenic bacteriaSingle nucleotide polymorphism detectionSpore displayStreptavidinWilson's disease

Abstract 

Aims: The purpose of this study was to develop a general method for the facile development of a new DNA biosensor which utilizes streptavidin-displayed spores as a molecular machinery. Methods and Results: Fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of Bacillus thuringiensis spores and as a diagnosis method for DNA detection. As a proof-of-concept, four pathogenic bacteria including Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli and Klebsiella pneumonia were used for the detection of pathogenic species. In addition, a set of mutant variants of Wilson's disease were also used for the detection of single nucleotide polymorphism (SNP) in this system. Conclusions: This strategy, utilizing streptavidin-displayed spores, is capable of capturing DNA targets for the detection of pathogenic bacteria and for mutation analysis in Wilson's disease. Significance and Impact of the Study: This approach could be useful as a simple platform for developing sensitive spore-based biosensors for any desired DNA targets in diagnostic applications. ? 2011 The Authors. Letters in Applied Microbiology

ISSN 

0266-8254

Link 

http://dx.doi.org/10.1111/j.1472-765X.2011.03131.x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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