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Title 

Effect of Bcl-xL overexpression on erythropoietin production in recombinant Chinese hamster ovary cells treated with dimethyl sulfoxide

Authors 

Yeon Gu KimJ Y KimByoung-Woo ParkJungoh AhnJoon Ki JungHong-Weon LeeG M LeeEun Gyo Lee

Publisher 

Elsevier

Issue Date 

2011

Citation 

Process Biochemistry, vol. 46, no. 11, pp. 2201-2204

Keywords 

ApoptosisBcl-xLDimethyl sulfoxideInducible expressionrCHO cells

Abstract 

Dimethyl sulfoxide (DMSO) can increase the specific productivity (q) of foreign proteins in mammalian cells, while it can also induce cell death, particularly apoptosis. Bcl-xL is a typical anti-apoptotic protein that inhibits the apoptosis in recombinant Chinese hamster ovary (rCHO) cell culture. To evaluate the potential role of Bcl-xL overexpression on DMSO-mediated erythropoietin (EPO) production, we used EPO-producing rCHO cells with regulated Bcl-xL overexpression (EPO-off-Bcl-xL) by doxycycline. Although DMSO addition enhanced specific EPO productivity (qEPO), it also induced cell death in EPO-off-Bcl-xL cells. Bcl-xL overexpression reduced the DMSO-induced cell death followed by release of various enzymes from plasma membrane-damaged cells as evidenced from LDH assay, resulting in delayed loss of EPO. However, it did not significantly improve the maximum EPO production. In addition, Bcl-xL overexpression suppressed DMSO-induced apoptosis, characterized by DNA fragmentation and Annexin V staining. Taken together, Bcl-xL overexpression could inhibit DMSO-induced apoptosis, thereby delaying the loss of EPO.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2011.07.017

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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