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Title 

Adjuvant effect of bacterial outer membrane vesicles with penta-acylated lipopolysaccharide on antigen-specific T cell priming

 

T세포 활성화를 촉진하는 변형 LPS를 보유한 세균외막 소포체의 애쥬번트 효능

Authors 

D H LeeSang-Hyun KimW KangY S ChoiSang Ho LeeSang Rae LeeS YouH K LeeKyu Tae ChangE C Shin

Publisher 

Elsevier

Issue Date 

2011

Citation 

Vaccine, vol. 29, no. 46, pp. 8293-8301

Keywords 

AdjuvantOuter membrane vesiclesPenta-acylated LPST cells

Abstract 

Outer membrane vesicles (OMV) are nano-sized spherical blebs shed by Gram-negative bacteria and have been utilized in vaccine development. In the present study, we evaluated T cell adjuvant activity of OMV with strictly penta-acylated LPS produced by Δ msbB/Δ pagP mutant of non-pathogenic Escherichia coli W3110 (mOMV) compared to OMV with hexa-acylated LPS produced by wild-type E. coli W3110 (wOMV). Penta-acylation of LPS renders mOMV less endotoxic than wOMV in in vitro and in vivo toxicity assays. In mice, mOMV has adjuvant activity on T cell priming not only in KLH protein immunization but also in SIINFEKL peptide immunization. The T-cell adjuvant activity of mOMV was comparable to that of wOMV and LPS and was abrogated in TLR4 K/O mice. In innate immunity, mOMV stimulated BMDCs to up-regulate co-stimulatory and antigen-presenting molecules and to produce pro-inflammatory cytokines in a TLR4-dependent manner. Of note, mOMV induced cytokine production at a significantly less extent compared with wOMV. Taken together, we propose that mOMV with penta-acylated LPS is a safe vaccine adjuvant for T cell priming and can be used in vaccine development against viral diseases and cancer.

ISSN 

0264-410X

Link 

http://dx.doi.org/10.1016/j.vaccine.2011.08.102

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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