상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

An expressed sequence tag analysis for the fast-growing shoots of Bambusa edulis Murno

 

빠른 생장속도를 나타내는 대나무 죽순의 EST 분석

Authors 

E ChoY UmS K YooH LeeH B KimKoh SerryH C ShinY Lee

Publisher 

The Botanical Society of Korea

Issue Date 

2011

Citation 

Journal of Plant Biology, vol. 54, no. 6, pp. 402-408

Keywords 

BamboocDNA libraryReal-time RT-PCRShoot growth

Abstract 

Bamboo is one of the fastest growing plants in the world and is an economically important crop species in Asia. To identify the genes involved in fast shoot growth, an expressed sequence tag analysis was performed on Bambusa edulis Murno fast-growing shoots. Sequencing of the cDNA clones generated 1,402 5′-end high-quality expressed sequence tags (JG296384-JG297785, average length 655 bp), of which 1,101 clusters (143 consensus and 958 singletons) were revealed by sequence comparison to be unique and 597 (54% of total clusters) of them have a putative ATG start codon. A Basic Local Alignment Search Tool X analysis showed that 995 of these genes were similar to genes present in the National Center for Biotechnology database. A total of 868 genes were most similar to rice genes. The most abundant genes were three thionin-coding genes, which have 27, 17, or 10 clones, respectively, followed by aminocyclopropanecarboxylate oxidase and cysteine protease. Thionin and putative cell elongation-associated genes, xyloglucan endotransglycosylase/hydrolase, expansin, cellulose synthase, and pectin esterase were analyzed by real-time reverse transcription polymerase chain reaction using gene-specific primers. These results suggest that this high-quality library could be a good resource for understanding molecular events of bamboo shoot elongation, and the full-length clones could be used for crop improvement studies in the future.

ISSN 

1226-9239

Link 

http://dx.doi.org/10.1007/s12374-011-9179-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)