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Title 

Rapid concentration of some bacteriocin-like compounds using an organic solvent

Authors 

Dong Min ChungK E KimSeong Yoep JeongC S ParkKeug Hyun AhnD H KimD O KangHyo Kon ChunByung Dae YoonH B KohH J KimN S Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Food Science and Biotechnology, vol. 20, no. 5, pp. 1457-1459

Keywords 

anti-microbialBacillus cereusbacteriocin-like compoundorganic solvent

Abstract 

A new solvent extraction method for isolation of bacteriocin-like compounds (BLCs) from bacterial culture broth was developed. Culture supernatant of Pediococcus acidilactici WRL-1 was mixed with 3 volumes of cold acetone and maintained at ?20°C for 2 h. The solvent and precipitate were separated by centrifugation. Most (95.2 ±2.5%) pediocin activity was found in the liquid phase and it was obtained by evaporation. This method was also an effective means of isolating other BLCs from cultures of Lactobacillus acidophilus, Lactobacillus plantarum, and Bacillus licheniformis DJ-2. Other solvents (methanol, ethanol, and butanol) were also suitable toward this end.

ISSN 

1226-7708

Link 

http://dx.doi.org/10.1007/s10068-011-0201-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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