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Title 

Visualizing cellular dynamics in plant-microbe interactions using fluorescent-tagged proteins

 

GFP 형광단백질을 활용한 식물병원균 감염에 대한 식물세포내 다양한 반응 관찰방법

Authors 

W UnderwoodSerry KohS C Somerville

Publisher 

Humana Press

Issue Date 

2011

Citation 

Methods in Molecular Biology, vol. 712, no. 0, pp. 283-291

Abstract 

Interactions between plant cells and microbial pathogens involve highly dynamic processes of cellular trafficking and reorganization. Substantial advances in imaging technologies, including the discovery and widespread use of fluorescent proteins as tags as well as advances in laser-based confocal microscopy have provided the first glimpses of the dynamic nature of the processes of defense and pathogenicity. Prior to the development of these techniques, high resolution imaging by electron microscopy gave only a static picture of these dynamic events and live cell imaging was significantly limited in resolution as well as the availability of relevant stains and markers. The incorporation of fluorescent protein fusions and laser-based confocal microscopy into studies of plant-microbe interactions has opened the door to fascinating new questions about the cellular response to attempted infection. Additionally, studies of cellular responses to pathogen infection may lead to new knowledge about fundamental processes in plant cells, such as mechanisms underlying subcellular trafficking and targeting of proteins and other molecules.

ISSN 

1064-3745

Link 

http://dx.doi.org/10.1007/978-1-61737-998-7_21

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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