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Title 

Effect of nitrogen limitation on oleic acid biosynthesis in Botryococcus braunii

Authors 

Gang Guk ChoiByung Hyuk KimChi-Yong AhnHee-Mock Oh

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Journal of Applied Phycology, vol. 23, no. 6, pp. 1031-1037

Keywords 

BiodieselBotryococcus brauniiNitrogen limitationOleic acidsadStearoyl-ACP desaturase

Abstract 

The influence of nitrogen (N) deficiency on the cell growth and intracellular lipid production of the alga Botryococcus braunii UTEX 572 was investigated. Biomass concentration and lipid content of B. braunii cultivated in modified Chu-13 medium containing 0.04, 0.37, and 3.66 mM nitrate were 0.23-0.38 g L-1 and 36-63% of dry cell weight, respectively. The specific growth rate of B. braunii reached a constant of 0.185 day-1 during cultivation with an initial nitrate feed of 3.66 mM. The maximum lipid content of B. braunii was 63% with 0.04 mM nitrate. However, the maximum lipid productivity of 0.019 g L-1 day-1 was achieved with 0.37 mM nitrate. The level of oleic acid, an important component of biodiesel, was higher at 86% of the total fatty acids under N-limited conditions (0.04 mM nitrate) compared to 69% under N-sufficient conditions (3.66 mM nitrate). Furthermore, expression of the stearoyl-ACP desaturase gene (sad) encoding a stearoyl-ACP desaturase involved in the synthesis of oleic acid was 2. 6-fold higher under N-limited conditions than under N-sufficient conditions.

ISSN 

0921-8971

Link 

http://dx.doi.org/10.1007/s10811-010-9636-1

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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