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Title 

EST analysis predicts putatively causative genes underlying the pharmaceutical application of Glycyrrhiza uralensis fisch

Authors 

Dong-Wook KimRyong Nam KimSang-Haeng ChoiD W KimSeong-Hyeuk NamH S ChoiH D KohAe-Ri KimS H ChaeJ C AhnAram KangHong-Seog Park

Publisher 

Springer Verlag (Germany)

Issue Date 

2011

Citation 

Plant Molecular Biology Reporter, vol. 29, no. 4, pp. 814-824

Keywords 

ESTGlycyrrhiza uralensis FischglycyrrhizinPharmaceutical application

Abstract 

Glycyrrhiza uralensis Fisch is an important medicinal plant used not only as a natural sweetener but also for a variety of pharmaceutical applications. Even though there have been previous reports of genes putativelyassociated with the biosynthesis of the sweet compound glycyrrhizin, the absence of genomic information and the insufficient transcriptomic data for this plant still hinder sufficient insight into the causative genes underlying the pharmaceutical and culinary applications of Glycyrrhiza uralensis Fisch. In this study, we sequenced 5,276 ESTs from Glycyrrhiza uralensis Fisch, established 2,353 unique sequences based on clustering and performed comparative analysis of them. Of the 2,353 unique sequences, we found homologs and orthologs for 1,499 of them with similarity searches against the non-redundant protein database at NCBI using the BLASTX algorithm and against the InterPro database using hidden Markove model (HMM) methods. We have functionally classified 749 unique sequences using Gene Ontology (GO) terms, mapped pathways for 248 unique sequences with the Kyoto Encyclopedia of Genes and Genomes (KEGG) and predicted 30 secreted proteins. Based on their expression levels, we have identified genesencoding a novel beta-D-xylosidase, glutathione-S-transferase and arabinogalactan protein 16 that may function in the glycyrrhizin biosynthesis, anti-tumor activity and detoxifying activity of this very promising medicinal plant. Furthermore, the EST sequence information described in this paper could be a valuable resource for future scientific research projects on this plant, including genome sequencing and annotation and genetic diversity as well as functional genomics and molecular functional studies.

ISSN 

0735-9640

Link 

http://dx.doi.org/10.1007/s11105-011-0290-9

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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