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Title 

Production of 3-hydroxypropionic acid through propionaldehyde dehydrogenase PduP mediated biosynthetic pathway in Klebsiella penumoniae

Authors 

L H LuoChul Ho KimSun-Yeon HeoBaek Rock OhWon Kyung HongSeonghun KimD H KimJeong-Woo Seo

Publisher 

Elsevier

Issue Date 

2012

Citation 

Bioresource Technology, vol. 103, no. 1, pp. 1-6

Keywords 

3-hydroxypropionic acidGlycerolKlebsiella pneumoniaePropanediol utilization proteinPropionaldehyde dehydrogenase

Abstract 

The pduP gene encodes a propionaldehyde dehydrogenase (PduP) was investigated for the role in 3-hydroxypropionic acid (3-HP) glycerol metabolism in Klebsiella pneumoniae. The enzyme assay showed that cell extracts from a pduP mutant strain lacked measurable dehydrogenase activity. Additionally, the mutant strain accumulated the cytotoxic intermediate metabolite 3-hydroxypropionaldehyde (3-HPA), causing both cell death and a lower final 3-HP titer. Ectopic expression of pduP restored normal cell growth to mutant. The enzymatic property of recombinant protein from Escherichia coli was examined, exhibiting a broad substrate specificity, being active on 3-HPA. The present work is thus the first to demonstrate the role of PduP in glycerol metabolism and biosynthesis of 3-HP.

ISSN 

0960-8524

Link 

http://dx.doi.org/10.1016/j.biortech.2011.09.099

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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