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Title 

Expression of the protective antigen for PEDV in transgenic duckweed, Lemna minor

 

형질전한 좀개구리밥에서 PEDV antigen 발현

Authors 

S M KoH J SunMyung Jin OhI J SongM J KimH S SinC H GohY W KimP O LimH Y LeeSuk Weon Kim

Publisher 

Korean Society for Horticultural Science

Issue Date 

2011

Citation 

Horticulture, Environment, and Biotechnology, vol. 52, no. 5, pp. 511-515

Keywords 

aquatic plantporcine diarrheaspike proteintransformationvaccine

Abstract 

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

Citation 

ISSN 

0253-6498

Link 

http://dx.doi.org/10.1007/s13580-011-0007-x

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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