상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Enhancement of ethanol production from glycerol in a Klebsiella pneumoniae mutant strain by the inactivation of lactate dehydrogenase

Authors 

Baek Rock OhJeong-Woo SeoSun-Yeon HeoWon Kyung HongL H LuoSeonghun KimOh Suk KwonJung Hoon SohnM H JoeD H ParkChul Ho Kim

Publisher 

Elsevier

Issue Date 

2012

Citation 

Process Biochemistry, vol. 47, no. 1, pp. 156-159

Keywords 

EthanolGlycerolKlebsiella pneumoniaeLactate dehydrogenase

Abstract 

Previously, using γ-irradiation treatment, we isolated a mutant strain of Klebsiella pneumoniae (named GEM167) that showed high-level ethanol production from glycerol. In the present study, in an effort to enhance ethanol production, we used a deletion of the lactate dehydrogenase gene to engineer a mutant strain incapable of lactate synthesis. In the ΔldhA mutant of GEM167, the production of ethanol was significantly increased from 21.5 g/l to 28.9 g/l and from 0.93 g/(l h) to 1.2 g/(l h). Introduction of the Zymomonas mobilis pdc and adhII genes encoding pyruvate decarboxylase and aldehyde dehydrogenase, respectively, further improved the ethanol production level from glycerol to 31.0 g/l; this is the highest level reported to date.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/j.procbio.2011.10.011

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)