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Title 

Regulatory roles of ganglioside GQ1b in neuronal cell differentiation of mouse embryonic stem cells

 

갱글리오사이드의 GQ1b에 의해 마우스 줄기세포에서 신경세포로의 분화를 조절

Authors 

D H KwakJ W JinJ S RyuK KoS D LeeJeong Woong LeeJi Su KimK Y JungJ Y MaK A HwangKyu Tae ChangY K Choo

Publisher 

Korean Society for Biochemistry and Molecular Biology

Issue Date 

2011

Citation 

BMB Reports, vol. 44, no. 12, pp. 799-804

Keywords 

8-sialyltransferase 5 (ST8Sia5)Ganglioside GQ1bMAP kinaseMouse embryonic stem cells (mESCs)Neuronal differentiationST8 alpha-N-acetyl-neuraminidase alpha-2

Abstract 

Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is elated to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway. Gangliosides play an important role in neuronal differentiation processes. The regulation of ganglioside levels is elated to the induction of neuronal cell differentiation. In this study, the ST8Sia5 gene was transfected into mESCs and then differentiated into neuronal cells. Interestingly, ST8Sia5 gene transfected mESCs expressed GQ1b by HPTLC and immunofluorescence analysis. To investigate the effects of GQ1b over-expression in neurogenesis, neuronal cells were differentiated from GQ1b expressing mESCs in the presence of retinoic acid. In GQ1b expressing mESCs, increased EBs formation was observed. After 4 days, EBs were co-localized with GQ1b and nestin, and GFAP. Moreover, GQ1b co-localized with MAP-2 expressing cells in GQ1b expressing mESCs in 7-day-old EBs. Furthermore, GQ1b expressing mESCs increased the ERK1/2 MAP kinase pathway. These results suggest that the ST8Sia5 gene increases ganglioside GQ1b and improves neuronal differentiation via the ERK1/2 MAP kinase pathway.

ISSN 

1976-6696

Link 

http://dx.doi.org/10.5483/BMBRep.2011.44.12.799

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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