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Title | Preparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography |
Authors | G H Quan; Sei Ryang Oh; Hyuk Hwan Song; Hyeong Kyu Lee; Y W Chin |
Publisher | Korean Society for Applied Biological Chemisty |
Issue Date | 2011 |
Citation | Journal of Korean Society for Applied Biological Chemistry, vol. 54, no. 4, pp. 623-627 |
Keywords | 8-methoxypsoralen; High-speed counter-current chromatography; Pulsatilla chinensis |
Abstract | A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis. |
ISSN | 1738-2203 |
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Appears in Collections | |
Registered Date |
2017-04-19 |
