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Title 

Preparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography

Authors 

G H QuanSei Ryang OhHyuk Hwan SongHyeong Kyu LeeY W Chin

Publisher 

Korean Society for Applied Biological Chemisty

Issue Date 

2011

Citation 

Journal of Korean Society for Applied Biological Chemistry, vol. 54, no. 4, pp. 623-627

Keywords 

8-methoxypsoralenHigh-speed counter-current chromatographyPulsatilla chinensis

Abstract 

A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.

ISSN 

1738-2203

Link 

http://dx.doi.org/10.3839/jksabc.2011.094

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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