상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Fermentation strategies for 1,3-propanediol production from glycerol using a genetically engineered Klebsiella pneumoniae strain to eliminate by-product formation

Authors 

Baek Rock OhJeong-Woo SeoSun-Yeon HeoWon Kyung HongL H LuoJ H SonD H ParkChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Bioprocess and Biosystems Engineering, vol. 35, no. 1, pp. 159-165

Keywords 

1,3-PropanediolBy-product formationGlycerolKlebsiella pneumoniaeNutrient co-supplementationTwo-stage fermentation

Abstract 

We generated a genetically engineered Klebsiella pneumoniae strain (AK-VOT) to eliminate by-product formation during production of 1,3-propanediol (1,3-PD) from glycerol. In the present study, the glycerol-metabolizing properties of the recombinant strain were examined during fermentation in a 5 L bioreactor. As expected, by-product formation was completely absent (except for acetate) when the AK-VOT strain fermented glycerol. However, 1,3-PD productivity was severely reduced owing to a delay in cell growth attributable to a low rate of glycerol consumption. This problem was solved by establishing a two-stage process separating cell growth from 1,3-PD production. In addition, nutrient co-supplementation, especially with starch, significantly increased 1,3-PD production from glycerol during fed-batch fermentation by AK-VOT in the absence of by-product formation.

ISSN 

1615-7591

Link 

http://dx.doi.org/10.1007/s00449-011-0603-2

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)