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Title 

Production of cellulase enzymes during the solid-state fermentation of empty palm fruit bunch fiber

Authors 

Seonghun KimChul Ho Kim

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Bioprocess and Biosystems Engineering, vol. 35, no. 1, pp. 61-67

Keywords 

CellulaseCellulosomeEmpty palm fruit bunch fiberFungusLignocellulosic biomassPenicillium verruculosumSolid-state fermentation

Abstract 

Penicillium verruculosum COKE4E is a fungal strain isolated from bituminous coal. The microorganism cultivated in a minimal medium supplemented with Avicel, carboxymethylcellulose, and oat spelt xylan produced cellulase enzymes as exhibiting carboxymethylcellulase (CMCase), Avicelase, xylanase, and cellobiosidase activities. In this study, the productivity of the extracellular enzymes in the strain was evaluated by using empty palm fruit bunch fiber (EPFBF), a lignocellulosic biomass, as a substrate for solid-state bioconversion. The highest cellulase activities were observed after 6 days of fermentation at pH 6.0 and 30 °C. The enzymes were secreted as cellulosomes for the degradation of EPFBF as a sole carbon source. Focused ion beam analysis showed that P. verruculosum COKE4E produced cellulolytic enzymes that were able to effectively biodegrade EPFBF during solid-state fermentation. In this process, 6.5 U of CMCase, 6.8 U of Avicelase, and 8.8 U of xylanase per gram of dry solid EPFBF were produced. These results demonstrate that EPFBF may be a potential raw material in solid-state fermentation for the production of cellulase enzymes to be used for biofuel production.

ISSN 

1615-7591

Link 

http://dx.doi.org/10.1007/s00449-011-0595-y

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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