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Title 

A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-κB signaling pathway in RAW 264.7 macrophages

 

워크리아의 항염증 활성

Authors 

Ji Won ParkOk-Kyoung KwonHa Young JangHyouk JoungSei Ryang OhHyeong Kyu LeeS B HanKyung Seop Ahn

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Inflammation, vol. 35, no. 1, pp. 321-331

Keywords 

InflammationMAPKNF-κBNitric oxideWercklea insignis

Abstract 

The biological activity of Wercklea insignis (WI) in inflammation and the underlying mechanisms of action of extracts of this plant are largely unknown. In the present study, we investigated the effects of a WI methanolic extract on lipopolysaccharide-stimulated inflammation in the mouse macrophage cell line, RAW 264.7. A WI methanolic extract significantly inhibited NO, PGE2, IL-6, IL-1β, and TNF-α production in LPS-stimulated RAW 264.7 cells. Expression of iNOS, COX-2, IL-6, IL-1β, and TNF-α were suppressed by the extract at both the mRNA and protein levels in lipopolysaccharide (LPS)-stimulated cells. Additionally, the attenuation of inflammatory responses in RAW 264.7 cells by the WI extract was closely associated with suppression of phosphorylation of mitogen-activated protein kinase (MAPK) molecules, including ERK, JNK1/ 2, and p38 MAPK and translocation of the nuclear factor (NF)-κB p65 subunit into the nucleus. The effect of WI extract was investigated against carrageenan-induced paw edema in female (20-25 g). Our results collectively indicate that the WI extract inhibits LPS-induced inflammatory responses by blocking the NF-κB signaling pathway in macrophages, supporting use of the extract as a therapeutic anti-inflammatory treatment.

ISSN 

0360-3997

Link 

http://dx.doi.org/10.1007/s10753-011-9322-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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