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Title 

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

 

치료용 단백질 생산을 위한 CHO세포의 연구 성과와 미래 연구 방향

Authors 

J Y KimYeon Gu KimG M Lee

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Applied Microbiology and Biotechnology, vol. 93, no. 3, pp. 917-930

Keywords 

Cell engineeringCell line developmentCHO cellsOmics-based approachesVector engineering

Abstract 

Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins. Recent advances in cell culture technology for rCHO cells have achieved significant improvement in protein production leading to titer of more than 10 g/L to meet the huge demand from market needs. This achievement is associated with progression in the establishment of high and stable producer and the optimization of culture process including media development. In this review article, we focus on current strategies and achievements in cell line development, mainly in vector engineering and cell engineering, for high and stable protein production in rCHO cells. The approaches that manipulate various DNA elements for gene targeting by site-specific integration and cis-acting elements to augment and stabilize gene expression are reviewed here. The genetic modulation strategy by "direct" cell engineering with growth-promoting and/or productivity-enhancing factors and omics-based approaches involved in transcriptomics, proteomics, and metabolomics to pursue cell engineering are also presented.

ISSN 

0175-7598

Link 

http://dx.doi.org/10.1007/s00253-011-3758-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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