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Title 

Widdrol induces apoptosis via activation of AMP-activated protein kinase in colon cancer cells

Authors 

Moorim KangS K ParkChang Woo LeeIg Jun ChoYeong Nang JoJeong Wook YangJin-Ah KimJi Eun YunKi Hoon LeeH J KwonB W KimK LeeJong Soon KangH M Kim

Publisher 

Spandidos Publications

Issue Date 

2012

Citation 

Oncology Reports, vol. 27, no. 5, pp. 1407-1412

Keywords 

AMP-activated protein kinaseApoptosisColon cancerWiddrol

Abstract 

Widdrol, a natural sesquiterpene present in Juniperus sp., has been shown to exert anticancer and antifungal effects. Emerging evidence has suggested that AMP-activated protein kinase (AMPK), which functions as a cellular energy sensor, is a potential therapeutic target for human cancers. In this study, we found that AMPK mediates the anticancer effects of widdrol through induction of apoptosis in HT-29 colon cancer cells. We showed that widdrol induced the phosphorylation of AMPK in a dose- and time-dependent manner. The selective AMPK inhibitor compound C abrogated the inhibitory effect of widdrol on HT-29 cell growth. In addition, we demonstrated that widdrol induced apoptosis and this was associated with the activation of caspases, including caspase-3/7 and caspase-9, in HT-29 cells. We also demonstrated that transfection of HT-29 cells with AMPK siRNAs significantly suppressed the widdrol-mediated apoptosis and the activation of caspases. However, cell cycle arrest induced by widdrol was not affected by transfection of HT-29 cells with AMPK siRNAs. Furthermore, widdrol inhibited HT-29 tumor growth in a human tumor xenograft model. Taken together, our results suggest that the anticancer effect of widdrol may be mediated, at least in part, by induction of apoptosis via AMPK activation. -------------------------------------------------------------------------------- Reaxys Database Information

ISSN 

1021-335X

Link 

http://dx.doi.org/10.3892/or.2012.1644

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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