상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Molecular basis of the fructose-2,6-bisphosphatase reaction of PFKFB3: Transition state and the C-terminal function

Authors 

M C CavalierSong-Gun KimD NeauY H Lee

Publisher 

Wiley-Blackwell

Issue Date 

2012

Citation 

Proteines, vol. 80, no. 4, pp. 1143-1153

Keywords 

CatalysisConformationGlycolysisTransition stateX-ray crystallography --------------------------------------------------------------------------------

Abstract 

The molecular basis of fructose-2,6-bisphosphatase (F-2,6-P 2ase) of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB) was investigated using the crystal structures of the human inducible form (PFKFB3) in a phospho-enzyme intermediate state (PFKFB3-P?F-6-P), in a transition state-analogous complex (PFKFB3?AlF 4), and in a complex with pyrophosphate (PFKFB3?PP i) at resolutions of 2.45, 2.2, and 2.3 ?, respectively. Trapping the PFKFB3-P?F-6-P intermediate was achieved by flash cooling the crystal during the reaction, and the PFKFB3?AlF 4 and PFKFB3?PP i complexes were obtained by soaking. The PFKFB3?AlF 4 and PFKFB3?PP i complexes resulted in removing F-6-P from the catalytic pocket. With these structures, the structures of the Michaelis complex and the transition state were extrapolated. For both the PFKFB3-P formation and break down, the phosphoryl donor and the acceptor are located within ∼5.1 ?, and the pivotal point 2-P is on the same line, suggesting an "in-line" transfer with a direct inversion of phosphate configuration. The geometry suggests that NE2 of His253 undergoes a nucleophilic attack to form a covalent N-P bond, breaking the 2O-P bond in the substrate. The resulting high reactivity of the leaving group, 2O of F-6-P, is neutralized by a proton donated by Glu322. Negative charges on the equatorial oxygen of the transient bipyramidal phosphorane formed during the transfer are stabilized by Arg252, His387, and Asn259. The C-terminal domain (residues 440-446) was rearranged in PFKFB3?PP i, implying that this domain plays a critical role in binding of substrate to and release of product from the F-2,6-P 2ase catalytic pocket. These findings provide a new insight into the understanding of the phosphoryl transfer reaction.

ISSN 

0887-3585

Link 

http://dx.doi.org/10.1002/prot.24015

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)