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Title 

Monitoring of horizontal gene transfer from agricultural microorganisms to soil bacteria and analysis of microbial community in soils

 

농업용 미생물로부터 토양 세균으로 수평적인 유전자 이동의 모니터링과 토양 내 미생물 군집의 분석

Authors 

Sung Eun KimJae Sun MoonWon Sik ChoiS H LeeSung Uk Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2012

Citation 

Journal of Microbiology and Biotechnology, vol. 22, no. 4, pp. 563-566

Keywords 

Agricultural microorganismHorizontal gene transferMicrobial communitySoil bacteriaT-RFLP --------------------------------------------------------------------------------

Abstract 

To investigate the possibility of horizontal gene transfer between agricultural microorganisms and soil microorganisms in the environment, Bacillus subtilis KB producing iturin and the PGPR recombinant strain Pseudomonas fluorescens MX1 were used as model microorganisms. The soil samples of cucumber or tomato plants cultivated in pots and the greenhouse for a six month period were investigated by PCR, real-time PCR, Southern hybridization, and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting. Our data from Southern blotting and TRFLP patterns suggest that the model bacteria do not give significant impacts on the other bacteria in the pots and greenhouse during cultivation.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1110.10066

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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