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Title 

Heat shock protein gene family of the Porphyra seriata and enhancement of heat stress tolerance by PsHSP70 in Chlamydomonas

 

모무늬 돌김의 Heat shock protein 유전자발굴 및 PsHSP70 유전자 발현 클라미도모나스에서 고온 내성 증가

Authors 

H S ParkWon Joong JeongE KimY JungJong Min LimM S HwangE J ParkD S HaD W Choi

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Marine Biotechnology, vol. 14, no. 3, pp. 332-342

Keywords 

ChlamydomonasHeat toleranceHSPPorphyra seriata

Abstract 

Heat shock proteins and molecular chaperones are key components contributing to survival in the abiotic stress response. Porphyra seriata grows on intertidal rocks exposed to dynamic environmental changes associated with the turning tides, including desiccation and heat stress. Analysis of the ESTs of P. seriata allows us to identify the nine HSP cDNAs, which are predicted to be PsHSP90, three PsHSP70, PsHSP40 and PsHSP20, and three 5′-truncated HSP cDNAs. RT-PCR results show that most of the PsHSP transcripts were detected under normal cell growth conditions as well as heat stress, with the exception of two cDNAs. In particular, PsHSP70b and PsHSP20 transcripts were upregulated by heat stress. When the putative mitochondrial PsHSP70b was introduced and overexpressed in Chlamydomonas, transformed Chlamydomonas evidenced higher rates of survival and growth than those of the wild type under heat stress conditions. Constitutive overexpression of the PsHSP70b gene increases the transcription of the HSF1 as well as the CrHSP20 and CrHSP70 gene. These results indicate that PsHSP70b is involved in tolerance to heat stress and the effects on transcription of the CrHSP20 and CrHSP70 genes.

ISSN 

1436-2228

Link 

http://dx.doi.org/10.1007/s10126-011-9417-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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