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Title 

Phytosterols play a key role in plant innate immunity against bacterial pathogens by regulating nutrient efflux into the apoplast

Authors 

K WangM Senthil-KumarChoong-Min RyuL KangK S Mysore

Publisher 

American Society of Plant Biologists

Issue Date 

2012

Citation 

Plant Physiology, vol. 158, no. 4, pp. 1789-1802

Abstract 

Bacterial pathogens colonize a host plant by growing between the cells by utilizing the nutrients present in apoplastic space. While successful pathogens manipulate the plant cell membrane to retrieve more nutrients from the cell, the counteracting plant defense mechanism against nonhost pathogens to restrict the nutrient efflux into the apoplast is not clear. To identify the genes involved in nonhost resistance against bacterial pathogens, we developed avirus-induced gene-silencing-based fastforward genetics screen in Nicotiana benthamiana. Silencing of N. benthamiana SQUALENE SYNTHASE, a key gene in phytosterol biosynthesis, not only compromised nonhost resistance to few pathovars of Pseudomonas syringae and Xanthomonas campestris, but also enhanced the growth of the host pathogen P. syringae pv tabaci by increasing nutrient efflux into the apoplast. An Arabidopsis (Arabidopsis thaliana) sterol methyltransferase mutant (sterol methyltransferase2) involved in sterol biosynthesis also compromised plant innate immunity against bacterial pathogens. The Arabidopsis cytochrome P450 CYP710A1, which encodes C22-sterol desaturase that converts b-sitosterol to stigmasterol, was dramatically induced upon inoculation with nonhost pathogens. An Arabidopsis Atcyp710A1 null mutant compromised both nonhost and basal resistance while overexpressors of AtCYP710A1 enhanced resistance to host pathogens. Our data implicate the involvement of sterols in plant innate immunity against bacterial infections by regulating nutrient efflux into the apoplast.

ISSN 

0032-0889

Link 

http://dx.doi.org/10.1104/pp.111.189217

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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