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Title 

Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system

 

E. coli strain과 유도제의 조합에 의한 글루칸 합성효소의 대량발현

Authors 

Jin A KoHyung Jae JeongYoung Bae RyuSu-Jin ParkY J WeeD KimYoung Min KimWoo Song Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2012

Citation 

Journal of Microbiology and Biotechnology, vol. 22, no. 4, pp. 510-515

Keywords 

DextransucraseLactoseLeuconostoc citreum KM20OptimizationResponse surface methodology --------------------------------------------------------------------------------

Abstract 

A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD 600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17°C. These results may effectively apply to the heterologous expression of other large DexT genes.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1111.11032

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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