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Title 

Effective screening of Scenedesmus sp. from environmental microalgae communities using optimal sonication conditions predicted by statistical parameters of fluorescence-activated cell sorting

Authors 

Hyun Joon LaJae Yon LeeSong-Gun KimGang Guk ChoiChi-Yong AhnHee-Mock Oh

Publisher 

Elsevier

Issue Date 

2012

Citation 

Bioresource Technology, vol. 114, no. 0, pp. 478-483

Keywords 

Coefficient of variationEnvironmental microalgae communitiesFACSScenedesmus sp.Sonication

Abstract 

The effects of the sonication parameters, including the power and time, were investigated for the effective isolation of Scenedesmus sp. from environmental microalgae communities when using fluorescence-activated cell sorting (FACS). The selectivity, defined as the percentage of Scenedesmus sp. successfully isolated and grown in microplates, appeared as peaks in contour plots spanned by the sonication power and time. For fast screening of the optimal sonication conditions, correlations between the selectivity and the statistical parameters from the FACS analysis were investigated. A graphical comparison analysis of the contour plots showed a pattern similarity of over 82% between the coefficients of variation for the side scatter (SSC-CV) and the selectivity. This predictability of the optimal sonication conditions enabled a Scenedesmus sp. selectivity of ca. 2. times using only one-third of the sonication condition sets arbitrarily chosen around the peaks of the SSC-CV, thereby saving resources and time for subsequent processes.

ISSN 

0960-8524

Link 

http://dx.doi.org/10.1016/j.biortech.2012.02.140

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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