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Title 

Protein tyrosine phosphatase profiling analysis of HIB-1B cells during brown adipogenesis

Authors 

Hye-Ryung ChoiWon Kon KimEun Young KimHyeyun JungJeong Hoon KimBaek Soo HanK H YouSang Chul LeeKwang-Hee Bae

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2012

Citation 

Journal of Microbiology and Biotechnology, vol. 22, no. 7, pp. 1029-1033

Keywords 

Brown adipogenesisProtein tyrosine phosphatasePTP-REPTPome --------------------------------------------------------------------------------

Abstract 

A number of evidence have been accumulated that the regulation of reversible tyrosine phosphorylation, which can be regulated by the combinatorial activity of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs), plays crucial roles in various biological processes including differentiation. There are a total of 107 PTP genes in the human genome, collectively referred to as the "PTPome." In this study, we performed PTP profiling analysis of the HIB-1B cell line, a brown preadipocyte cell line, during brown adipogenesis. Through RT-PCR and real-time PCR, several PTPs showing differential expression pattern during brown adipogenesis were identified. In the case of PTP-RE, it was shown to decrease significantly until 4 days after brown adipogenic differentiation, followed by a dramatic increase at 6 days. The overexpression of PTP-RE led to decreased brown adipogenic differentiation via reducing the tyrosine phosphorylation of the insulin receptor, indicating that PTP-RE functions as a negative regulator at the early stage of brown adipogenesis.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1112.12059

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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