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Title 

Gene expression profiles analyzed by DNA sequencing of cDNA clones constructed from porcine preadipocytes and adipocytes

Authors 

D M LeeP BajracharyaE J JangE J LeeS H ChaeHong-Seog ParkS W KangY S LeeK H LeeJ ChangC S ChungI Choi

Publisher 

The Genetics Society of Korea

Issue Date 

2012

Citation 

Genes & Genomics, vol. 34, no. 2, pp. 125-131

Keywords 

AdipogenesiscDNA libraryESTGene expressionPorcine

Abstract 

As a step towards understanding the molecular mechanism of adipogenesis in pigs, preadipocytes purified from the back fat of 1 day-old female piglets were used for in vitro culture. Normalized cDNA libraries were constructed with 1.6×10 7 and 1.1×10 7 independent clones from preadipocyte and mature adipocyte mRNAs, respectively. Polymerase chain reaction (PCR) result using primers T3 and T7 (universal primer) confirmed the presence of the insert in the vector. Sequencing of 2,112 randomly selected clones from each cDNA library identified 217 clusters, 1,169 singletons, and 216 contigs in preadipocytes and 231 clusters, 1,100 singletons, and 233 contigs in mature adipocytes. Expressed sequence tag (EST) identified 24 genes with known annotation highly expressed in adipocytes and 21 in preadipocytes by at least four EST number. Among those 45 genes, when analyzed by real time RT-PCR, 76% of the gene showed significant difference between preadipocytes and mature adipocytes. Highly expressed genes in mature adipocytes were related to adipogenesis, extracellular matrix control and oncogenes, whereas cytoskeleton-related genes were down-regulated. An interesting similarity found during gene profile studies indicated a correlation between cancer and adipogenesis.

ISSN 

1976-9571

Link 

http://dx.doi.org/10.1007/s13258-011-0075-4

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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