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Title 

Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli

Authors 

Soo-Young ParkSeung Hwan ParkSoo Keun Choi

Publisher 

Elsevier

Issue Date 

2012

Citation 

Analytical Biochemistry, vol. 426, no. 1, pp. 63-65

Keywords 

Active inclusion bodyPaenibacillus polymyxaPoxBRecombinant protein expression

Abstract 

Overexpression of Paenibacillus polymyxa PoxB in Escherichia coli induced the formation of inclusion bodies. An enzyme assay showed that the inclusion bodies exhibited PoxB activity, indicating that they were biologically active. Fusion of GFP and Bacillus subtilis AmyE to the C-terminus of the PoxB also induced the formation of biologically active aggregates when they were overexpressed in E. coli. Therefore, P. polymyxa PoxB can be used as a fusion partner to promote the formation of active inclusion bodies in E. coli.

ISSN 

0003-2697

Link 

http://dx.doi.org/10.1016/j.ab.2012.04.002

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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