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Title 

Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase

 

덱스트란수크라제를 활용한 퓨라닌 글루코사이드의 효소적 합성

Authors 

Jin-A KoYoung Bae RyuTae Soon ParkHyung Jae JeongJang Hoon KimSu-Jin ParkJoong Su KimD KimYoung Min KimWoo Song Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2012

Citation 

Journal of Microbiology and Biotechnology, vol. 22, no. 9, pp. 1224-1229

Keywords 

DextransucraseLeuconostoc lactisPuerarinTransglucosylationWater solubility

Abstract 

Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.

ISSN 

1017-7825

Link 

http://dx.doi.org/10.4014/jmb.1202.02007

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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