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Title 

Inhibition of collagen deposit in obstructed rat bladder outlet by transplantation of superparamagnetic iron oxide-labeled human mesenchymal stem cells as monitored by molecular magnetic resonance imaging (MRI)

Authors 

H J LeeJ H WonS H DooJ H KimK Y SongS J LeeI LimKyu Tae ChangY S SongS U Kim

Publisher 

Cognizant Communication Corporation

Issue Date 

2012

Citation 

Cell Transplantation, vol. 21, no. 5, pp. 959-970

Keywords 

(TGF-β)Bladder outlet obstructionCell transplantationCollagenHuman mesenchymal stem cells (MSCs)Magnetic resonance imaging (MRI)Superparamagnetic iron oxide nanoparticle (SPION)Transforming growth factor-β --------------------------------------------------------------------------------

Abstract 

Bladder outlet obstruction (BOO) caused by collagen deposit is one of the most common problems in elderly males. The present study is to investigate if human mesenchymal stem cells (MSCs) are capable of inhibiting collagen deposition and improve cystometric parameters in bladder outlet obstruction in rats. Human MSCs were labeled with nanoparticles containing superparamagnetic iron oxide (SPION), and transplanted in rat BOO lesion site. Forty 6-week-old female Sprague-Dawley rats were divided into four groups (group 1: control, group 2: sham operation, group 3: BOO, and group 4: BOO rats receiving SPION-hMSCs). Two weeks after the onset of BOO, 1 × 10 6 SPION-hMSCs were injected into the bladder wall. Serial T2- weighted MR images were taken immediately after transplantation of SPION-labeled human MSCs and at 4 weeks posttransplantation. T2-weighted MR images showed a clear hypointense signal induced by the SPION-labeled MSCs. While the expression of collagen and TGF-β protein increased after BOO, the expression of both returned to the original levels after MSC transplantation. Expression of HGF and c-met protein also increased in the group with MSC transplantation. Maximal voiding pressure and residual urine volume increased after BOO but they recovered after MSC transplantation. Human MSCs transplanted in rat BOO models inhibited the bladder fibrosis and mediated recovery of bladder dysfunction. Transplantation of MSCbased cell therapy could be a novel therapeutic strategy against bladder fibrosis in patients with bladder outlet obstruction.

ISSN 

0963-6897

Link 

http://dx.doi.org/10.3727/096368911X627516

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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