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Title 

Large-scale transcriptome sequencing and gene analyses in the crab-eating macaque (macaca fascicularis) for biomedical research

 

게잡이 원숭이의 대량 전사체 및 유전자 분석

Authors 

Jae Won HuhYoung Hyun KimSang Je ParkDae Soo KimSang Rae LeeKyeong Min KimJi Su KimBong Seok SongBo Woong SimSun-Uk KimSang-Hyun KimKyu Tae Chang

Publisher 

BioMed Central

Issue Date 

2012

Citation 

BMC Genomics, vol. 13, no. 0, pp. 163-163

Abstract 

Background: As a human mimic, the crab-eating macaque (Macaca fascicularis) is an invaluable non-human primate model for biomedical research, but the lack of genetic information on this primate has represented a significant obstacle for its broader use. Results: Here, we sequenced the transcriptome of 16 tissues and identified genes to resolve the main obstacles for understanding the biological response of the crab-eating macaque. From 4 million reads with 1.4 billion base sequences, 31,786 isotigs containing genes similar to those of humans, 12,672 novel isotigs, and 348,160 singletons were identified using the GS FLX sequencing method. Approximately 86% of human genes were represented among the genes sequenced in this study. Additionally, 175 tissue-specific genes were identified, 81 of which were experimentally validated. In total, 4,314 alternative splicing (AS) events were identified and analyzed. Intriguingly, 10.4% of AS events were associated with transposable element (TE) insertions. Finally, investigation of TE exonization events and evolutionary analysis were conducted, revealing interesting phenomena of human-specific amplified trends in TE exonization events. Conclusions: This report represents the first large-scale transcriptome sequencing and genetic analyses of M. fascicularis and could contribute to its utility for biomedical research and basic biology.

ISSN 

1471-2164

Link 

http://dx.doi.org/10.1186/1471-2164-13-163

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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