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Title 

Non-ionic polysorbate surfactants: alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for production of extracellular MCL-PHA depolymerases

Authors 

B S SeoDo Young KimY Y NiKwang Hee SonHo Yong ParkY H Rhee

Publisher 

Elsevier

Issue Date 

2012

Citation 

Bioresource Technology, vol. 121, no. 1, pp. 47-53

Keywords 

MCL-PHAMedium-chain-length poly(3-hydroxyalkanoate) depolymerasePolysorbate surfactantsPseudomonas alcaligenes LB19Tween compounds

Abstract 

The potential of non-ionic polysorbate surfactants as alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for the production of diverse bacterial MCL-PHA depolymerases was evaluated. When grown with corn oil as the sole carbon substrate, Pseudomonas alcaligenes LB19 preferentially produced lipolytic enzymes, but its MCL-PHA depolymerase was not induced by the substrate. However, the results of activity staining and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis clearly revealed that Tween 20 induced simultaneous production of lipolytic enzymes and the MCL-PHA depolymerase with the molecular mass (26.5. kDa) of P. alcaligenes LB19, which has been previously identified. Moreover, the co-production of two functionally distinct hydrolytic enzymes induced by Tween 20 was commonly observed in various Gram-positive and Gram-negative bacteria that were fed the substrate. Thus, it is expected that non-ionic polysorbate surfactants including Tween 20 can be widely exploited as promising universal substrates for the facile and efficient production of diverse MCL-PHA depolymerases.

ISSN 

0960-8524

Link 

http://dx.doi.org/10.1016/j.biortech.2012.06.118

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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