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Title 

Expression of a recombinant chimeric protein of human colorectal cancer antigen GA733-2 and Fc fragment of antibody using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves

Authors 

K I KimH Y ChungK H YooJ H ParkH H LeeY J SeokK S KoH S KangKyung Jin LeeDoo-Byoung OhY H JoungI S Chung

Publisher 

Springer Verlag (Germany)

Issue Date 

2012

Citation 

Plant Biotechnology Reports, vol. 6, no. 3, pp. 233-242

Keywords 

BCTV (Beet curly top virus)Colorectal cancerGA733-2Nicotiana benthamianaVaccination

Abstract 

We describe expression and characterization of recombinant human colorectal cancer antigen GA733-2 fused to Fc fragment of antibody (GA733-2-Fc) using a replicating vector based on Beet curly top virus in infiltrated Nicotiana benthamiana leaves. Recombinant GA733-2-Fc/KDEL with a molecular mass of ~68 kDa was transiently expressed. The level of expression of GA733-2-Fc with ER retention signal KDEL (GA733-2-Fc/KDEL) in the expression vector system was 0. 96% of total soluble proteins. Recombinant GA733-2-Fc/KDEL was purified using an affinity chromatography. Mice immunized with recombinant GA733-2-Fc/KDEL mounted a strong GA733-2-Fc/KDEL-specific serum antibody response. Vaccination of plant-derived recombinant GA733-2-Fc/KDEL regressed tumor volumes in BALB/c mice. The population of activated-T and NK-T cells increased notably in lymph node, spleen, and tumor-infiltrating lymphocytes derived from the tumor-regressed mice. Taken together, recombinant GA733-2-Fc/KDEL expressed in plants can be used as an effective experimental immunogen for research in cancer vaccine development.

ISSN 

1863-5466

Link 

http://dx.doi.org/10.1007/s11816-012-0218-3

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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