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Title 

Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)

Authors 

Minhwa DoWon-Gu JangJ H HwangHoon JangEun Jung KimEun Jeong JeongH ShimS S HwangK B OhS J ByunJ H KimJeong Woong Lee

Publisher 

Elsevier

Issue Date 

2012

Citation 

Biochemical and Biophysical Research Communications, vol. 424, no. 3, pp. 765-770

Keywords 

Mitochondrial DNARecloned pigSomatic cell nuclear transfer

Abstract 

Somatic cell nuclear transfer (SCNT) has been established for the transmission of specific nuclear DNA. However, the fate of donor mitochondrial DNA (mtDNA) remains unclear. Here, we examined the fate of donor mtDNA in recloned pigs through third generations. Fibroblasts of recloned pigs were obtained from offspring of each generation produced by fusion of cultured fibroblasts from a Minnesota miniature pig (MMP) into enucleated oocytes of a Landrace pig. The D-loop regions from the mtDNA of donor and recipient differ at nucleotide sequence positions 16050 (A→T), 16062 (T→C), and 16135 (G→A). In order to determine the fate of donor mtDNA in recloned pigs, we analyzed the D-loop region of the donor's mtDNA by allele-specific PCR (AS-PCR) and real-time PCR. Donor mtDNA was successfully detected in all recloned offspring (F1, F2, and F3). These results indicate that heteroplasmy that originate from donor and recipient mtDNA is maintained in recloned pigs, resulting from SCNT, unlike natural reproduction.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1016/j.bbrc.2012.07.031

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2019-05-02


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